Liberatori S, Bini L, De Felice C, Magi B, Marzocchi B, Raggiaschi R, Pallini V, Bracci R
Cattedra di Neonatologia, Università degli Studi di Siena, Italy.
Electrophoresis. 1997 Mar-Apr;18(3-4):520-6. doi: 10.1002/elps.1150180331.
Plasma from eight newborns (4 pre-term and 4 full-term) with early-onset (< 72 h) sepsis and six apparently healthy controls was analyzed. The presence of spots identified as haptoglobin and serum amyloid A protein was the electrophoretic result most consistently associated with disease. Time course monitoring showed rises, peaks and declines of spot intensity as expected for acute-phase proteins induced by transient stimuli. Haptoglobin beta chains appear to be undersialated in pre-term newborns, whereas post-translational modifications of alpha chains and serum amyloid A protein are similar to those observed in adults. The undersialation of beta chain and occurrence of alpha chain phenotypes different from those found in maternal serum indicate that perinatal haptoglobin originates from neonatal synthesis.
对8名患有早发性（<72小时）败血症的新生儿（4名早产儿和4名足月儿）以及6名明显健康的对照者的血浆进行了分析。电泳结果显示，被鉴定为触珠蛋白和血清淀粉样蛋白A的斑点的存在与疾病最一致相关。时间进程监测显示，斑点强度的上升、峰值和下降符合由短暂刺激诱导的急性期蛋白的预期情况。早产儿的触珠蛋白β链似乎唾液酸化不足，而α链和血清淀粉样蛋白A的翻译后修饰与成人中观察到的相似。β链的唾液酸化不足以及与母体血清中不同的α链表型的出现表明，围产期触珠蛋白源自新生儿合成。
