Bini L, Magi B, Marzocchi B, Cellesi C, Berti B, Raggiaschi R, Rossolini A, Pallini V
Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Italy.
Electrophoresis. 1996 Mar;17(3):612-6. doi: 10.1002/elps.1150170333.
Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of alpha 1-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As the the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.
急性期血清蛋白通过在3 - 10固定pH梯度下进行等电聚焦的二维电泳进行分析。大多数斑点通过参考SWISS - 2DPAGE数据库中的血浆图谱来鉴定。血清淀粉样蛋白A蛋白斑点通过用特异性抗血清进行免疫印迹以及通过与电泳参数预测值匹配来鉴定。α1 - 抗胰蛋白酶、富含亮氨酸糖蛋白、触珠蛋白、血清视黄醇结合蛋白和转甲状腺素蛋白浓度的变化通过银染凝胶的光密度测定进行定量。比较了18例细菌性疾病患者和16例病毒性疾病患者的电泳图谱。血清淀粉样蛋白A蛋白斑点的发生率在细菌性疾病中为18/18，在病毒性疾病中为6/16。作为所研究的其他反应物，细菌性疾病中的变化是同时发生的，而在病毒性疾病中则倾向于交错发生。
