Moore J W, Millard S, Babidge W, Rowland R, Roediger W E
Cell Physiology Laboratory, University of Adelaide Department of Surgery, Queen Elizabeth Hospital, Australia.
Aust N Z J Surg. 1997 May;67(5):245-9. doi: 10.1111/j.1445-2197.1997.tb01956.x.
Several lines of evidence suggest a possible role for reduced forms of sulphur (including sulphide) in ulcerative colitis. The aims of this study were to assess the metabolic profile of colonic epithelial cells after treatment in vivo with hydrogen sulphide and correlate this with mucosal histological appearances.
Adult Sprague-Dawley rats had antegrade Roux-en-Y colostomies fashioned to allow access to the 'in-flow' bowel. Animals were treated with 2 mL sodium hydrosulphide (10, 20, 30 mmol/L) or saline control twice daily via the stoma for four (acute experiments) and 90 (chronic experiments) days. Isolated colonic epithelial cell suspensions prepared from such animals were incubated in the presence of [1-14C]-labelled n-butyrate (5 mmol/L) or [6-14C]glucose (5 mmol/L). Metabolic performance was measured radiometrically (14CO2 production) and enzymatically (ketone body production and lactogenesis). The histological appearances of treated mucosa were scored for acute inflammatory changes.
There was a highly significant reduction in 14CO2 production from both n-butyrate and glucose in all groups compared to the control in both acute and chronic experiments. There was no difference between groups with respect to histological appearance and no evidence of acute inflammation in any specimen.
Sodium hydrosulphide impairs rat colonic epithelial metabolic performance in vivo, but does not produce mucosal inflammation.
多项证据表明，还原态硫（包括硫化物）在溃疡性结肠炎中可能发挥作用。本研究旨在评估硫化氢体内治疗后结肠上皮细胞的代谢谱，并将其与黏膜组织学表现相关联。
成年Sprague-Dawley大鼠行顺行性Roux-en-Y结肠造口术，以便能够接触到“流入”肠段。动物经造口每日两次接受2 mL硫化氢钠（10、20、30 mmol/L）或生理盐水对照处理，持续4天（急性实验）和90天（慢性实验）。从这些动物制备的分离结肠上皮细胞悬液在[1-14C]标记的正丁酸（5 mmol/L）或[6-14C]葡萄糖（5 mmol/L）存在的情况下进行孵育。通过放射性测量（14CO2产生）和酶促法（酮体产生和乳糖生成）测定代谢性能。对处理后黏膜的组织学表现进行急性炎症变化评分。
在急性和慢性实验中，与对照组相比，所有组中正丁酸和葡萄糖产生的14CO2均显著减少。各实验组在组织学表现上无差异，且任何标本均无急性炎症迹象。
硫化氢钠在体内损害大鼠结肠上皮代谢性能，但不引起黏膜炎症。
