纤细病毒科内玉米褪绿矮缩病毒的核苷酸序列与分类学

Nucleotide sequence and taxonomy of maize chlorotic dwarf virus within the family Sequiviridae.

作者信息

Reddick B B, Habera L F, Law M D

机构信息

University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, TN 37901-1071, USA.

出版信息

J Gen Virol. 1997 May;78 ( Pt 5):1165-74. doi: 10.1099/0022-1317-78-5-1165.

DOI:10.1099/0022-1317-78-5-1165

PMID:9152437

Abstract

The complete sequence of a Tennessee isolate of maize chlorotic dwarf virus (MCDV-TN) was determined from cDNA clones and by direct sequencing of the viral RNA. The genome is 11813 nucleotides (nt) in length and contains one large open reading frame between nt 435 and 10763 that encodes a polyprotein of 3443 amino acids. The N-terminal amino acid sequences were determined for the three capsid proteins. All three were adjacent, starting at nt 2526 and putatively ending at nt 3761. Comparison of the sequence of MCDV-TN with other viral sequences revealed similarities to several plant picorna-like viruses including members of the Sequiviridae, Comoviridae and Potyviridae. This work also provides evidence based on genome organization that MCDV-TN is a member of the genus Waikavirus within the family Sequiviridae.

摘要

从cDNA克隆和病毒RNA的直接测序确定了一株来自田纳西州的玉米褪绿矮缩病毒（MCDV-TN）的完整序列。该基因组长度为11813个核苷酸（nt），在nt 435和10763之间包含一个大的开放阅读框，编码一个由3443个氨基酸组成的多聚蛋白。测定了三种衣壳蛋白的N端氨基酸序列。所有三种蛋白相邻，起始于nt 2526，推测终止于nt 3761。将MCDV-TN的序列与其他病毒序列进行比较，发现它与几种植物类微小核糖核酸病毒相似，包括联体病毒科、豇豆花叶病毒科和马铃薯Y病毒科的成员。这项工作还基于基因组结构提供了证据，证明MCDV-TN是联体病毒科中Waikavirus属的成员。