Division of Genetics and Tree Improvement, Institute of Forest Biodiversity (ICFRE), Hyderabad, India.
Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, India.
Virus Genes. 2022 Dec;58(6):598-604. doi: 10.1007/s11262-022-01931-7. Epub 2022 Aug 30.
Secoviruses are mono-/bipartite plant-infecting, icosahedral RNA viruses that incite economically important diseases in plants. In the present study, nine secoviruses tentatively named as Ananas comosus secovirus (AcSV), Artocarpus altilis secovirus (AaSV), Boehmeria nivea secovirus (BnSV), Gynostemma pentaphyllum secovirus (GpSV), Orobanche cernua secovirus (OcSV), Paris polyphylla secovirus 1 (PpSV1), Paris polyphylla secovirus 2 (PpSV2), Rhododendron delavayi secovirus (RdSV), and Yucca gloriosa secovirus (YgSV) were identified by probing publicly available transcriptomes of eight plant species. Coding-complete genome/genome segments of all the identified viruses encoding a polyprotein were recovered. Two of the nine identified viruses-AcSV and GpSV were discovered in few of the small RNA libraries of respective plant species. Putative cleavage sites were predicted in polyproteins encoded by AcSV, GpSV, PpSV2 and YgSV genome segments. Phylogenetic and sequence identity analyses revealed that AcSV, GpSV and YgSV, PpSV1 and RdSV putatively belong to the genera- Sadwavirus (sub genus: Cholivirus), Fabavirus, Nepovirus and Waikavirus, respectively, while AaSV, BnSV, and PpSV2 may represent a distinct group of viruses within the family Secoviridae as they could not conclusively be assigned to a single genus.
香蕉束顶病毒、菠萝密茎溃疡病毒、苎麻嵌纹病毒、绞股蓝曲茎病毒、独脚金内脐蠕孢病毒、云南金钱草双生病毒 1、云南金钱草双生病毒 2、黄花鹤顶兰呼肠孤病毒和嘉兰叶状病毒是单分体/二分体植物侵染的二十面体 RNA 病毒，可引起植物的重要经济疾病。在本研究中，通过探测 8 种植物的公开转录组，鉴定了 9 种暂定名为菠萝密束顶病毒（AcSV）、菠萝密茎溃疡病毒（AaSV）、苎麻嵌纹病毒（BnSV）、绞股蓝曲茎病毒（GpSV）、独脚金内脐蠕孢病毒（OcSV）、云南金钱草双生病毒 1（PpSV1）、云南金钱草双生病毒 2（PpSV2）、黄花鹤顶兰呼肠孤病毒（RdSV）和嘉兰叶状病毒（YgSV）的病毒。从所有鉴定的病毒中回收了编码多聚蛋白的编码完整基因组/基因组片段。在各自植物物种的少数小 RNA 文库中发现了 9 种鉴定病毒中的 2 种，即 AcSV 和 GpSV。在 AcSV、GpSV、PpSV2 和 YgSV 基因组片段编码的多聚蛋白中预测了潜在的切割位点。系统发育和序列同一性分析表明，AcSV、GpSV 和 YgSV、PpSV1 和 RdSV 分别推测属于 Sadwavirus（Cholivirus 亚属）、Fabavirus、Nepovirus 和 Waikavirus 属，而 AaSV、BnSV 和 PpSV2 可能代表了一个独特的病毒群，因为它们不能明确地归属于科的一个属。
