Toga A W, Goldkorn A, Ambach K, Chao K, Quinn B C, Yao P
Department of Neurology, UCLA School of Medicine 90024-1769, USA.
Comput Med Imaging Graph. 1997 Mar-Apr;21(2):131-41. doi: 10.1016/s0895-6111(96)00072-9.
This study examined the densitometric and topographic detail of high resolution 3D digital postmortem cryosectioned brain images. Anatomic image data and histology from cryosectioned human brain were compared to in vivo MRI for the ability to delineate neuroanatomic structure. 3D surface reconstructions in the Talairach and Tournoux atlas ("Co-planar stereotaxic atlas of the human brain", Thieme, New York, 1988) coordinate system enabled morphometric comparisons for a representative sample of neuroanatomic structures. Spatial resolution of cryosection images averaged 200 and 170 microns/pixel for whole head and brain, respectively, and 40 microns/pixel for isolated the brain regions. Anatomic detail was far superior to MRI, particularly in deep subcortical regions such as the basal ganglia and in mesencephalic nuclei and tracts. Digital repositioning in the Talairach coordinate system enabled efficient structure localization and morphometric comparison. Histology from collected tissue sections provided cytologic detail that could be mapped to its approximate 3D context. This approach permits comprehensive morphometric analyses necessary for an anatomic framework to a digital atlas of the human brain.
本研究检测了高分辨率三维数字尸检冷冻切片脑图像的密度测定和地形细节。将冷冻切片人脑的解剖图像数据和组织学与活体MRI进行比较，以评估描绘神经解剖结构的能力。在Talairach和Tournoux图谱（《人脑共面立体定向图谱》，Thieme出版社，纽约，1988年）坐标系中的三维表面重建能够对神经解剖结构的代表性样本进行形态计量学比较。冷冻切片图像的空间分辨率，全脑和脑分别平均为200微米/像素和170微米/像素，分离的脑区为40微米/像素。解剖细节远优于MRI，尤其是在基底神经节等深部皮质下区域以及中脑核团和神经束。在Talairach坐标系中的数字重新定位能够实现有效的结构定位和形态计量学比较。收集的组织切片的组织学提供了细胞学细节，可将其映射到大致的三维背景中。这种方法允许对人脑数字图谱的解剖框架进行必要的综合形态计量学分析。
