Toga A W, Ambach K L, Schluender S
Laboratory of Neuro Imaging, UCLA School of Medicine 90024-1769, USA.
Neuroimage. 1994 Nov;1(4):334-44. doi: 10.1006/nimg.1994.1018.
We have generated a spatially accurate, high-resolution three-dimensional (3D) volume of brain anatomy from cryosectioned whole human head. The head of a female cadaver was cryosectioned on a heavy duty cryomacrotome (PMV, Stockholm Sweden) modified for quantitative digital image capture. Serial images (1024(2), 24-bit) were captured directly from the cryoplaned specimen blockface in 500-micron intervals and reconstructed to a 3D data volume. Data were placed into the Talairach coordinate system to create a volume of brain anatomy for atlas reference. We resampled the volume at 500 microns along the sagittal, coronal, and horizontal planes and enhanced the images by digitally editing the background. The spatial resolution of the original digitized images provided sufficient anatomic detail to clearly delineate gray and white matter and neural structures, including major fiber pathways, subthalamic nuclei, and laminae. We developed a compact disk and controlling software program to enable the viewer to select planes of orientation, display, and copy individual to sections at higher resolution. Animation proved useful in the conveyance of system anatomy as structures are shown traversing through the neuroaxis. Postmortem cryosectioning paired with this computerized presentation allowed the complete 3D volume data to be distributed and shared as an educational, clinical, and research resource.
我们从冷冻切片的完整人类头部生成了一个空间精确、高分辨率的三维（3D）脑解剖结构体积数据。一名女性尸体的头部在一台为定量数字图像采集而改装的重型冷冻切片机（瑞典斯德哥尔摩的PMV）上进行了冷冻切片。以500微米的间隔直接从冷冻切片标本的块表面采集系列图像（1024×1024，24位），并重建为三维数据体积。将数据放入Talairach坐标系中，以创建用于图谱参考的脑解剖结构体积数据。我们在矢状面、冠状面和水平面上以500微米的间隔对该体积数据进行重采样，并通过数字编辑背景来增强图像。原始数字化图像的空间分辨率提供了足够的解剖细节，能够清晰地勾勒出灰质、白质和神经结构，包括主要纤维束、丘脑底核和板层。我们开发了一个光盘和控制软件程序，使观察者能够选择定向平面、显示并以更高分辨率复制单个切片。动画在系统解剖学的展示中很有用，因为可以显示结构在神经轴上的穿行。死后冷冻切片与这种计算机化展示相结合，使得完整的三维体积数据能够作为教育、临床和研究资源进行分发和共享。
