Polette M, Gilles C, Marchand V, Lorenzato M, Toole B, Tournier J M, Zucker S, Birembaut P
INSERM U 314, IFR 53, CHU Maison Blanche, Reims, France.
J Histochem Cytochem. 1997 May;45(5):703-9. doi: 10.1177/002215549704500508.
Tumor cell-derived collagenase stimulatory factor (TCSF) stimulates in vitro the biosynthesis of various matrix metalloproteinases involved in tumor invasion, such as interstitial collagenase, gelatinase A, and stromelysin 1. The expression of TCSF mRNAs was studied in vivo, using in situ hybridization and Northern blotting analysis, in seven normal tissues and in 22 squamous cell carcinomas of the lung, and in seven benign proliferations and in 22 ductal carcinomas of the mammary gland. By in situ hybridization, TCSF mRNAs were detected in 40 of 44 carcinomas, in pre-invasive and invasive cancer cells of both lung and breast cancers. TCSF mRNAs and gelatinase A mRNAs were both visualized in the same areas in serial sections in breast cancers, and were expressed by different cells, tumor cells, and fibroblasts. The histological results were confirmed by Northern blot analysis, which showed a higher expression of TCSF mRNAs in cancers than in benign and normal tissues. These observations support the hypothesis that TCSF is an important factor in lung and breast tumor progression.
肿瘤细胞衍生的胶原酶刺激因子（TCSF）在体外可刺激多种参与肿瘤侵袭的基质金属蛋白酶的生物合成，如间质胶原酶、明胶酶A和基质溶解素1。利用原位杂交和Northern印迹分析，在体内研究了TCSF mRNA在7种正常组织、22例肺鳞状细胞癌、7种良性增生组织和22例乳腺导管癌中的表达情况。通过原位杂交，在44例癌症中的40例中检测到了TCSF mRNA，包括肺癌和乳腺癌的原位癌和浸润癌细胞。在乳腺癌的连续切片中，TCSF mRNA和明胶酶A mRNA在相同区域均有显示，且由不同细胞、肿瘤细胞和成纤维细胞表达。Northern印迹分析证实了组织学结果，该分析显示癌症中TCSF mRNA的表达高于良性和正常组织。这些观察结果支持了TCSF是肺和乳腺肿瘤进展中的一个重要因素这一假说。
