Liapis H, Hutton K
Department of Pathology, Washington University, St Louis, Missouri 63110, USA.
J Histochem Cytochem. 1997 May;45(5):737-41. doi: 10.1177/002215549704500512.
Integrins are heterodimeric transmembrane receptors, which are expressed in many cells. In vitro experiments have demonstrated that integrins may be important in tumor progression and organ development. The functions of integrins were previously studied in cell cultures and their tissue expression was detected by immunofluorescence or immunoperoxidase in frozen sections. The purpose of this study was to determine the optimal conditions for detection of integrins in formalin-fixed, paraffin-embedded tissues. We utilized microwave heating and enzyme digestion in routinely processed, surgically removed tissues. Our results demonstrate that integrins can be reliably detected in archival material. This approach will facilitate further investigation of the role played by integrins in human malignancies and in developmental processes.
整合素是异二聚体跨膜受体，在许多细胞中都有表达。体外实验表明，整合素可能在肿瘤进展和器官发育中起重要作用。整合素的功能此前是在细胞培养中进行研究的，其组织表达通过免疫荧光或免疫过氧化物酶在冰冻切片中进行检测。本研究的目的是确定在福尔马林固定、石蜡包埋组织中检测整合素的最佳条件。我们在常规处理的手术切除组织中采用了微波加热和酶消化方法。我们的结果表明，整合素能够在存档材料中得到可靠检测。这种方法将有助于进一步研究整合素在人类恶性肿瘤和发育过程中所起的作用。
