体内人眼泪pH值的荧光光度测量
Fluorophotometric measurement of pH of human tears in vivo.
作者信息
Yamada M, Mochizuki H, Kawai M, Yoshino M, Mashima Y
机构信息
Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
出版信息
Curr Eye Res. 1997 May;16(5):482-6. doi: 10.1076/ceyr.16.5.482.7050.
PURPOSE
To measure the pH in the precorneal tear film of humans in vivo using a pH-sensitive fluorescent dye, bis-carboxyethyl-carboxyfluorescein (BCECF).
METHODS
The measurement was initiated by instilling 1 microliter of 2 mM BCECF solution into the subject's eye. The pH was calculated by measuring the ratio of fluorescent intensities at two excitation wavelengths (490/430 ratio), which was dependent on pH, but independent of the dye concentration and other variables. The tears of the same subject were then collected and loaded on to a micro pH-meter to ensure the accuracy of the measurement.
RESULTS
The mean pH values of 40 eyes from 20 healthy volunteers was 7.50 (SD +/- 0.23), which corresponded well with those measured by the micro pH-meter.
CONCLUSIONS
The method described was useful in measuring the pH of the precorneal tear film of humans with minimal invasion.
目的
使用pH敏感荧光染料双羧乙基羧基荧光素（BCECF）在体内测量人类角膜前泪膜的pH值。
方法
通过向受试者眼中滴入1微升2 mM BCECF溶液开始测量。通过测量两个激发波长下的荧光强度比（490/430比值）来计算pH值，该比值取决于pH值，但与染料浓度和其他变量无关。然后收集同一受试者的眼泪并加载到微型pH计上以确保测量的准确性。
结果
20名健康志愿者的40只眼睛的平均pH值为7.50（标准差±0.23），与微型pH计测量的值非常吻合。
结论
所描述的方法对于以最小的侵入性测量人类角膜前泪膜的pH值很有用。
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