Cottet-Emard J M, Peyrin L
J Neural Transm. 1977;41(2-3):145-73. doi: 10.1007/BF01670279.
An optimized fluorimetric method is presented which permits the analysis of DOPA in urine or tissues, at endogenous levels. A wide variety of eluates can be analyzed by applying the manual or the automated schedule. The automated manifold, developed for DOPA assay, may be used to estimate norepinephrine (NE) in other samples, by only changing the nature of reagents to be pumped. Amounts of DOPA as low as 0.3 ng/ml of eluate can be detected. Determinations of endogenous DOPA are reported in urinary samples of Humans, Rats, Dogs and Sheeps, and in brain of Rats. The pattern of changes in DOPA urinary excretion has been investigated as a function of time in Rats. Dietary influences have been studied in Man, Rat and Dog. It is concluded that the greatest part of free and conjugated DOPA excreted in urine of these animals has an endogenous origin.
本文介绍了一种优化的荧光分析法，可对内源性水平的尿液或组织中的多巴进行分析。通过采用手动或自动程序，可对多种洗脱液进行分析。为多巴测定开发的自动流路，只需改变泵送试剂的性质，就可用于估计其他样品中的去甲肾上腺素（NE）。洗脱液中低至0.3 ng/ml的多巴量均可被检测到。本文报告了人类、大鼠、狗和绵羊尿液样本以及大鼠大脑中内源性多巴的测定结果。研究了大鼠尿液中多巴排泄量随时间变化的模式。还研究了人类、大鼠和狗的饮食影响。得出的结论是，这些动物尿液中排出的游离和结合多巴大部分来源于内源性。
