Masmiquel L, Castro-Forns M, de Torres I, García A, Vidal M T, Simó R
Department of Endocrinology, Hospital Universitari Vall d'Hebron, Barcelona, Spain.
J Clin Pathol. 1997 Feb;50(2):168-70. doi: 10.1136/jcp.50.2.168.
The aim was to evaluate Leu-M1 immunoreactivity as a prognostic factor in phaeochromocytoma. Anti-Leu-M1 monoclonal antibodies were used to determine the Leu-M1 immunoreactivity in 17 histologically confirmed phaeochromocytomas from 15 patients, using an avidin-biotin technique. Ten patients had a sporadic phaeochromocytoma, and five had multiple endocrine neoplasia type 2A (MEN 2A). Malignancy was diagnosed in three patients by the presence of metastases. Leu-M1 immunoreactivity was shown in 12 (70.5%) phaeochromocytomas. Three patterns of arrangement were observed: isolated (scattered positive cells) (n = 3); focal (aggregates of positive cells) (n = 5), and diffuse patterns (dispersed positive cells) (n = 4). Two cases of malignant phaeochromocytoma were positive (one focal and one isolated pattern). All cases of MEN 2A showed immunoreactivity, although no characteristic pattern was prevalent. A diffuse pattern was observed in all phaeochromocytomas longer than 7 cm. In conclusion, Leu-M1 expression is frequent in phaeochromocytoma. However, Leu-M1 immunoreactivity seems to be useless in predicting malignant behaviour and to be influenced mainly by tumour size.
目的是评估Leu-M1免疫反应性作为嗜铬细胞瘤预后因素的价值。采用抗生物素蛋白-生物素技术，运用抗Leu-M1单克隆抗体测定15例患者的17个经组织学确诊的嗜铬细胞瘤中的Leu-M1免疫反应性。10例患者患有散发性嗜铬细胞瘤，5例患有2A型多发性内分泌腺瘤病（MEN 2A）。3例患者因出现转移而被诊断为恶性肿瘤。12个（70.5%）嗜铬细胞瘤显示出Leu-M1免疫反应性。观察到三种排列模式：孤立型（散在阳性细胞）（n = 3）；局灶型（阳性细胞聚集）（n = 5）和弥漫型（分散阳性细胞）（n = 4）。2例恶性嗜铬细胞瘤呈阳性（1例局灶型和1例孤立型）。所有MEN 2A病例均显示免疫反应性，尽管没有普遍的特征性模式。在所有直径大于7 cm的嗜铬细胞瘤中均观察到弥漫型。总之，Leu-M1表达在嗜铬细胞瘤中很常见。然而，Leu-M1免疫反应性似乎在预测恶性行为方面并无用处，且主要受肿瘤大小影响。
