通过限制性片段长度多态性确认的因实验室交叉污染导致的结核分枝杆菌培养假阳性结果。
False-positive results from cultures of Mycobacterium tuberculosis due to laboratory cross-contamination confirmed by restriction fragment length polymorphism.
作者信息
Bauer J, Thomsen V O, Poulsen S, Andersen A B
机构信息
Department of Mycobacteriology, Statens Serum Institut, Copenhagen S, Denmark.
出版信息
J Clin Microbiol. 1997 Apr;35(4):988-91. doi: 10.1128/jcm.35.4.988-991.1997.
Abstract
During 1994, a cross-contamination problem leading to false-positive cultures of Mycobacterium tuberculosis was revealed in a mycobacteriology laboratory processing 30,000 to 35,000 samples per year. Molecular strain typing based on restriction fragment length polymorphism confirmed the contaminations. Out of 1,439 positive cultures of Mycobacterium tuberculosis, 49 samples from 48 patients were suspected to be cross-contaminated. In 37 cases, growth was observed both in BACTEC vials and on solid media, indicating that the contamination took place during the processing of the samples. The majority of the contaminated samples had been handled by the same technician.
摘要
1994年期间，在一个每年处理30000至35000份样本的分枝杆菌学实验室中，发现了一个导致结核分枝杆菌培养出现假阳性的交叉污染问题。基于限制性片段长度多态性的分子菌株分型证实了污染情况。在1439份结核分枝杆菌阳性培养物中，怀疑有来自48名患者的49份样本受到交叉污染。在37例病例中，在BACTEC瓶和固体培养基上均观察到生长，这表明污染发生在样本处理过程中。大多数受污染样本是由同一名技术人员处理的。
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