神经支配对体外培养的发育中肌肉乙酰胆碱敏感性的影响。
Effects of innervation on acetylcholine sensitivity of developing muscle in vitro.
作者信息
Betz W, Osborne M
出版信息
J Physiol. 1977 Aug;270(1):75-88. doi: 10.1113/jphysiol.1977.sp011939.
Abstract
- Chick embryo skeletal muscle fibres were grown in culture. The acetylcholine (AACh) sensitivity of non-innervated fibres was compared with that of fibres innervated in vitro by chick embryo ciliary ganglion neurones. 2. The general pattern of ACh sensitivity was unchanged by innervation: ACh hot spots were superimposed on a background of uniform ACh sensitivity. 3. Quantitative comparisons revealed two differences between non-innervated and innervated fibres. First, hot spots were encountered about one third more often on innervated fibres. Secondly, about one-third of the hot spots on innervated fibres had significantly higher ACh sensitivities than the remainder, which were similar to those on control fibres. 4. Apossible explanation of these results is that nerves which form synapses induce the appearanceof end-plates which have higher ACh sensitivities than the pre-existing ACh hot spots.
摘要
- 将鸡胚骨骼肌纤维进行体外培养。比较了未受神经支配的纤维与在体外由鸡胚睫状神经节神经元支配的纤维对乙酰胆碱（ACh）的敏感性。2. 神经支配并未改变ACh敏感性的总体模式：ACh热点叠加在均匀的ACh敏感性背景之上。3. 定量比较揭示了未受神经支配的纤维与受神经支配的纤维之间的两点差异。首先，在受神经支配的纤维上，热点出现的频率高出约三分之一。其次，受神经支配的纤维上约三分之一的热点对ACh的敏感性显著高于其余热点，而其余热点的敏感性与对照纤维上的热点相似。4. 对这些结果的一种可能解释是，形成突触的神经诱导出了终板的出现，这些终板对ACh的敏感性高于先前存在的ACh热点。
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