Christian C N, Daniels M P, Sugiyama H, Vogel Z, Jacques L, Nelson P G
Proc Natl Acad Sci U S A. 1978 Aug;75(8):4011-5. doi: 10.1073/pnas.75.8.4011.
There is an increase in the number of acetylcholine (AcCho) receptor aggregates on striated embryonic mouse myotubules when they are cocultured with clonal neuroblastoma-glioma hybrid cells. Medium conditioned by hybrid cells contains a factor which increases the number of AcCho receptor aggregates on myotubes cultured from mouse, rat or chick muscle. AcCho receptor-aggregating activity was present in medium conditioned by the neuroblastoma parent clone but was not detected in medium conditioned by cells of the parent glioma clone, fibroblasts, or HeLa cells. The factor increased the aggregation of AcCho receptors within 24 hr without a significant increase in the total number of AcCho receptors, and its action did not depend on myotube protein synthesis. The factor appears to rearrange the distribution of myotube AcCho receptors either by aggregating mobile AcCho receptors or by stabilizing labile receptor aggregates.
当横纹肌胚胎小鼠肌管与克隆性神经母细胞瘤-胶质瘤杂交细胞共培养时，其乙酰胆碱（AcCho）受体聚集体的数量会增加。杂交细胞条件培养基中含有一种因子，该因子可增加从小鼠、大鼠或鸡肌肉培养的肌管上AcCho受体聚集体的数量。神经母细胞瘤亲本克隆的条件培养基中存在AcCho受体聚集活性，但在亲本胶质瘤克隆、成纤维细胞或HeLa细胞的条件培养基中未检测到。该因子在24小时内增加了AcCho受体的聚集，而AcCho受体总数没有显著增加，并且其作用不依赖于肌管蛋白合成。该因子似乎通过聚集可移动的AcCho受体或稳定不稳定的受体聚集体来重新排列肌管AcCho受体的分布。
