Foulkes J A, Stewart D L
J Reprod Fertil. 1977 Sep;51(1):175-7. doi: 10.1530/jrf.0.0510175.
A lipoprotein separated from egg yolk by centrifugation and column chromatography was used to prepare a diluent containing 2-9% trisodium citrate. 2H2O (pH 7-0) and 7% glycerol. Ejaculates from Friesian bulls were split between this and two control diluents containing egg yolk, glycerol and citrate or lactose and used to inseminate once 1782 unselected Friesian cows. By 16 weeks after insemination with semen frozen in the egg yolk-lactose, egg yolk-citrate or lipoprotein-citrate diluents, 66-4, 67-1 and 66-1% of cows, respectively, had not returned to service.
通过离心和柱色谱法从蛋黄中分离出一种脂蛋白，用于制备一种含有2 - 9%柠檬酸三钠·2H₂O（pH 7.0）和7%甘油的稀释剂。从弗里生公牛采集的精液被分为三份，分别用这种稀释剂以及另外两种含有蛋黄、甘油和柠檬酸盐或乳糖的对照稀释剂进行处理，然后用于人工授精1782头未经挑选的弗里生奶牛。在用蛋黄 - 乳糖、蛋黄 - 柠檬酸盐或脂蛋白 - 柠檬酸盐稀释剂冷冻保存的精液进行人工授精16周后，分别有66.4%、67.1%和66.1%的奶牛未返情。
