Huhtanen P, Brotz P G, Satter L D
U.S. Dairy Forage Research Center, USDA, ARS, Madison, WI 53706, USA.
J Anim Sci. 1997 May;75(5):1380-92. doi: 10.2527/1997.7551380x.
A procedure allowing digesta sampling from the omasum via a ruminal cannula without repeated entry into the omasum was developed. The sampling system consisted of a device inserted into the omasum via the ruminal cannula, a tube connecting the device to the ruminal cannula, and a single compressor/vacuum pump. Eight cows given ad libitum access to a total mixed diet were used in a crossover design to evaluate the effects of the sampling system on digestive activity, animal performance, and animal behavior. Results indicated that the omasal sampling system has minimal effect on normal digestive and productive functions of high-producing dairy cows. Dry matter intake was reduced (24.0 vs 21.8 kg/d; P < .02) and seemed related more to the sampling procedures than to the device in the omasum. Observations of animal behavior indicated that cows with the sampling device were similar to control cows, although rumination and total chewing times were reduced slightly. The composition of digesta samples was biased toward an over-abundance of the liquid phase, but using a double-marker system to calculate digesta flow resulted in fairly small coefficients of variation for measurements of ruminal digestion variables. This technique may prove useful for partitioning digestion between the fermentative portion of the forestomach and the lower gastrointestinal tract. The omasal sampling procedure requires less surgical intervention than the traditional methods using abomasal or duodenal cannulas as sampling sites to study forestomach digestion and avoids potentially confounding endogenous secretions of the abomasum.
开发了一种通过瘤胃瘘管从瓣胃采集食糜样本的方法，无需反复进入瓣胃。采样系统由通过瘤胃瘘管插入瓣胃的装置、连接该装置与瘤胃瘘管的管子以及一台单压缩机/真空泵组成。采用交叉设计，使用8头自由采食全混合日粮的奶牛来评估采样系统对消化活动、动物生产性能和动物行为的影响。结果表明，瓣胃采样系统对高产奶牛的正常消化和生产功能影响极小。干物质摄入量减少（24.0对21.8千克/天；P < 0.02），且似乎更多与采样程序有关，而非与瓣胃中的装置有关。对动物行为的观察表明，装有采样装置的奶牛与对照奶牛相似，尽管反刍和总咀嚼时间略有减少。食糜样本的成分偏向液相过多，但使用双标记系统计算食糜流量，瘤胃消化变量测量的变异系数相当小。这项技术可能对区分前胃发酵部分和下消化道的消化作用有用。与使用皱胃或十二指肠瘘管作为采样部位来研究前胃消化的传统方法相比，瓣胃采样程序所需的手术干预更少，并且避免了皱胃潜在的内源性分泌物干扰。
