Yabushita H, Yoshikawa K, Hirata M, Furuya H, Hojyoh T, Fukatsu H, Noguchi M, Nakanishi M
Department of Obstetrics and Gynecology, Aichi Medical University, Nagakute-cho, Japan.
Gynecol Oncol. 1997 May;65(2):297-303. doi: 10.1006/gyno.1997.4659.
Electrochemotherapy is a new approach to the treatment of tumors that takes advantage of the permeabilization of the cell membranes by electric pulses to facilitate the intracellular delivery of chemotherapeutic drugs into cells. Using the female genital squamous cell carcinoma cell line, CaSki, the antitumor effectiveness of electrochemotherapy was tested. In vitro studies showed that the cytotoxicity of some anticancer drugs, especially bleomycin, can be greatly enhanced by exposing cells to noncytotoxic electric pulses. This enhancement was significantly greater when the electric treatment was given after exposure to the drug than when applied preexposure. Treatment of nude mice bearing subcutaneous transplanted tumors with noneffective intraperitoneal doses of bleomycin followed by local delivery of electric pulses similar to those performed in vitro resulted in tumor reduction and complete disappearance after 12 days this electrochemotherapy. Histological changes in tumor tissue such as necrosis and degeneration were extensive even 6 hr after the electrochemotherapy. Thus the antitumor effects of bleomycin in mice can be considerably potentiated by local electric pulses, suggesting that electrochemotherapy with bleomycin may have promise for treatment of vulvar or ectocervical squamous cell carcinomas with early invasion.
电化学疗法是一种治疗肿瘤的新方法，它利用电脉冲使细胞膜通透性增加，以促进化疗药物向细胞内递送。使用女性生殖系统鳞状细胞癌细胞系CaSki测试了电化学疗法的抗肿瘤效果。体外研究表明，通过将细胞暴露于无细胞毒性的电脉冲下，某些抗癌药物（尤其是博来霉素）的细胞毒性可大大增强。当在药物暴露后进行电处理时，这种增强作用比在暴露前应用时显著更大。用无效的腹腔内剂量博来霉素处理携带皮下移植瘤的裸鼠，然后进行类似于体外实验的局部电脉冲递送，12天后肿瘤缩小并完全消失。即使在电化学疗法后6小时，肿瘤组织中的坏死和变性等组织学变化仍很广泛。因此，局部电脉冲可显著增强博来霉素对小鼠的抗肿瘤作用，这表明博来霉素电化学疗法可能有望用于治疗早期浸润性外阴或宫颈鳞状细胞癌。
