Lu J H, Chiu Y T, Sung H W, Hwang B, Chong C K, Chen S P, Mao S J, Yang P Z, Chang Y
Homograft Laboratory, Veterans General Hospital-Taipei, Taiwan, R.O.C.
J Mol Cell Cardiol. 1997 Apr;29(4):1189-94. doi: 10.1006/jmcc.1996.0354.
This study sought to evaluate the use of tetrazolium salt XTT reduction as an indicator of valvular viability in a cryoprocessed porcine cardiac homograft model. The XTT tetrazolium assays was based on the metabolic reduction of Sodium 3'-[1-(phenylamino-carbonyl)-3,4-Tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate. The relationship between XTT reduction and: (1) leaflet tissue with various weight (n = 24); (2) morphometric evaluation (n = 30); (3) cadaveric ischemic intervals (n = 30); (4) freeze-thawing (n = 30) has been studied. The measurement of XTT reduction were significantly correlated with the weight of cardiac leaflets, in the range of 30 to 180mg (y=0.015x-0.063; r=0.99). Compared to morphometry of valvular damage, the reduction of mitochondrial enzymatic activity in cardiac leaflets was correlated with matrix cells without irreversible damage (r=0.89, P<0.005). The depletion of XTT reduction occurred dependent of ischemic time intervals. In general, freeze-thawing reduced more than 20% activity of mitochondrial dehydrogenase. We concluded that XTT tetrazolium assay is highly sensitive to determine valvular injury. The study demonstrated its potential for testing of cryopreserved cardiac valve.
本研究旨在评估在冷冻处理的猪心脏同种异体移植模型中，使用四氮唑盐XTT还原作为瓣膜活力指标的情况。XTT四氮唑测定基于3'-[1-(苯氨基羰基)-3,4-四氮唑]-双(4-甲氧基-6-硝基)苯磺酸钠水合物的代谢还原。研究了XTT还原与以下各项之间的关系：(1)不同重量的瓣叶组织（n = 24）；(2)形态学评估（n = 30）；(3)尸体缺血间隔时间（n = 30）；(4)冻融（n = 30）。XTT还原的测量与30至180mg范围内的心脏瓣叶重量显著相关（y = 0.015x - 0.063；r = 0.99）。与瓣膜损伤的形态学相比，心脏瓣叶中线粒体酶活性的降低与无不可逆损伤的基质细胞相关（r = 0.89，P < 0.005）。XTT还原的减少取决于缺血时间间隔。一般来说，冻融使线粒体脱氢酶活性降低超过20%。我们得出结论，XTT四氮唑测定对确定瓣膜损伤高度敏感。该研究证明了其在检测冷冻保存心脏瓣膜方面的潜力。
