Gajkowska B
Laboratory of the Ultrastructure of the Nervous System, Medical Research Centre Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1997;35(1):36-48.
The effect of transient, global ischemia on endothelin (ET) localization in CA1 area of hippocampus of the rats was investigated using post-embedding immunogold method. This paper provides immunocytochemical evidence that cerebral ischemia is accompanied by the increase of production of endothelin in CA1 area of the rat. This study focuses on the analysis ET-like immunoreactive neurons and glial cells in hippocampal CA1 area after long-term survival after ischemia: 1 week-12 months. One and two weeks after ischemia appearance of shrunken neurons and significant increase in ET-like immunoreactivity in astroglial cells as well as their proliferation was observed. Six and twelve months--marked immunoreactivity of endothelin in shrunken neurons and in perineuronal microglial macrophages was observed. The data suggest the possibility that the increased intracellular content of endothelin has a role in the development of neuronal death even following a long-term period after ischemia.
采用包埋后免疫金法研究短暂性全脑缺血对大鼠海马CA1区内皮素（ET）定位的影响。本文提供了免疫细胞化学证据，表明脑缺血伴随着大鼠CA1区内皮素产生的增加。本研究聚焦于分析缺血后长期存活（1周 - 12个月）的海马CA1区中内皮素样免疫反应性神经元和胶质细胞：缺血后1周和2周，观察到神经元萎缩，星形胶质细胞中内皮素样免疫反应性显著增加及其增殖。缺血后6个月和12个月，在萎缩的神经元和神经元周围的小胶质巨噬细胞中观察到内皮素的明显免疫反应性。数据表明，即使在缺血后很长一段时间，内皮素细胞内含量增加也可能在神经元死亡的发展中起作用。
