Gajkowska B, Mossakowski M J
Laboratory of Ultrastructure of Nervous System, Medical Research Centre, Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1995;33(4):221-30.
Electron microscopic immunocytochemical evaluation of localization and distribution of endothelins 1, 2 and 3 in the CA1 hippocampal sector in rats submitted to 10 min global cerebral ischemia was performed. The studies were done in different postischemic periods (10 min, 3, 6, 12 and 24 h) with postembedding immuno-gold technique for electron microscopy. Endothelin-like immunoreactivity was found in endothelial cells of hippocampal microvessels and in astrocytes, microglia, macrophages and in some axonal endings. The most pronounced changes appeared 24 h after ischemia. At that period all structural elements of blood-brain interphase: endothelium, basal membrane and perivascular astrocytic processes showed intensive endothelin-like immunoreactivity. Especially marked immunoreactivity was found in macrophages appearing in the proximity of microvessels. It has been stressed that ischemia-induced increased content of endothelin may play an important role in the pathogenesis of postischemic tissue abnormalities.
对经历10分钟全脑缺血的大鼠海马CA1区内皮素-1、内皮素-2和内皮素-3的定位和分布进行了电子显微镜免疫细胞化学评估。采用电子显微镜包埋后免疫金技术，在不同的缺血后时期（10分钟、3小时、6小时、12小时和24小时）进行研究。在海马微血管的内皮细胞、星形胶质细胞、小胶质细胞、巨噬细胞和一些轴突末梢中发现了内皮素样免疫反应性。最明显的变化出现在缺血后24小时。在那个时期，血脑界面的所有结构成分：内皮、基底膜和血管周围星形胶质细胞突起都显示出强烈的内皮素样免疫反应性。在微血管附近出现的巨噬细胞中发现了特别明显的免疫反应性。有人强调，缺血诱导的内皮素含量增加可能在缺血后组织异常的发病机制中起重要作用。
