Tang H, Gaietta G M, Fischer W H, Ellisman M H, Wong-Staal F
Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Science. 1997 May 30;276(5317):1412-5. doi: 10.1126/science.276.5317.1412.
A human nuclear protein that specifically interacts with the constitutive transport element (CTE) of simian retrovirus was identified as adenosine 5'-triphosphate-dependent RNA helicase A. This protein could bind to functional CTE but not to inactive CTE mutants. The interaction of helicase A with CTE was distinct from previously described helicase activity of this protein. Helicase A shuttled from the nucleus to the cytoplasm in the presence of a transcription inhibitor or in cells transiently overexpressing CTE-containing RNA. In vivo colocalization of helicase A and CTE was observed in experiments that combined in situ hybridization and immunostaining. These results suggest that helicase A plays a role in the nuclear export of CTE-containing RNA.
一种与猿猴逆转录病毒的组成型转运元件（CTE）特异性相互作用的人类核蛋白被鉴定为腺苷5'-三磷酸依赖性RNA解旋酶A。该蛋白可与功能性CTE结合，但不能与无活性的CTE突变体结合。解旋酶A与CTE的相互作用不同于该蛋白先前描述的解旋酶活性。在存在转录抑制剂的情况下或在瞬时过表达含CTE RNA的细胞中，解旋酶A从细胞核穿梭到细胞质。在原位杂交和免疫染色相结合的实验中观察到了解旋酶A和CTE在体内的共定位。这些结果表明解旋酶A在含CTE RNA的核输出中起作用。
