Iantsen E I, Ptitsyn A A, Filipenko M L, Batyrina O A, Muravlev A I, Mertvetsov N P
Genetika. 1997 Feb;33(2):243-8.
The possibility of using oligonucleotides from MER1 family repeats as PCR primers for the amplification of human genome DNA fragments was studied. The recommended oligonucleotide primers were chosen by computer analysis of the data base of the EMBL (release 37.0) nucleotide sequences. Use of one of the oligonucleotides was shown to allow specific human DNA amplification to be carried out.
研究了使用MER1家族重复序列的寡核苷酸作为PCR引物扩增人类基因组DNA片段的可能性。通过对EMBL(第37.0版)核苷酸序列数据库进行计算机分析,选择了推荐的寡核苷酸引物。结果表明,使用其中一种寡核苷酸能够进行特异性的人类DNA扩增。