寡核苷酸(GTG)5作为非结核分枝杆菌研究中的一种流行病学工具。
Oligonucleotide (GTG)5 as an epidemiological tool in the study of nontuberculous mycobacteria.
作者信息
Cilliers F J, Warren R M, Hauman J H, Wiid I J, van Helden P D
机构信息
MRC Centre for Molecular and Cellular Biology, Department of Medical Physiology and Biochemistry, University of Stellenbosch, Tygerberg, South Africa.
出版信息
J Clin Microbiol. 1997 Jun;35(6):1545-9. doi: 10.1128/jcm.35.6.1545-1549.1997.
Abstract
Analysis of restriction fragment length polymorphisms in the genome of Mycobacterium tuberculosis (DNA fingerprinting) has proved to be a useful epidemiological tool in the study of tuberculosis within populations or communities. However, to date, no similar method has been developed to study the epidemiology of nontuberculous mycobacteria (NTM). In this communication, we report that a simple oligonucleotide repeat, (GTG)5, can be used to accurately genotype all species and strains of NTM tested. We suggest that this technology is an easily applied and accurate tool which can be used for the study of the epidemiology of NTM.
摘要
结核分枝杆菌基因组中限制性片段长度多态性分析（DNA指纹图谱）已被证明是研究人群或社区内结核病的一种有用的流行病学工具。然而，迄今为止，尚未开发出类似方法来研究非结核分枝杆菌（NTM）的流行病学。在本报告中，我们报道了一种简单的寡核苷酸重复序列（GTG）5可用于准确地对所有测试的NTM菌种和菌株进行基因分型。我们认为该技术是一种易于应用且准确的工具，可用于NTM流行病学研究。
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