Collins D M, Erasmuson S K, Stephens D M, Yates G F, De Lisle G W
New Zealand Pastoral Agricultural Research Institute, Wallaceville Animal Research Centre, Upper Hutt.
J Clin Microbiol. 1993 May;31(5):1143-7. doi: 10.1128/jcm.31.5.1143-1147.1993.
Strains of Mycobacterium bovis, the causative organism of bovine tuberculosis, can be clearly distinguished from each other by restriction fragment analysis. This method of DNA fingerprinting has been used for many epidemiological studies in New Zealand, but the technique presents practical difficulties that hinder its widespread use. The insertion element IS6110 is being widely used as a DNA probe for distinguishing restriction fragment polymorphisms among strains of Mycobacterium tuberculosis. Both this element and another recently sequenced element, IS1081, are also present in M. bovis. We assessed the usefulness of these two elements for distinguishing between 160 strains of M. bovis. These strains, most of which were isolated in New Zealand, were selected to be representative of the 95 different types that were identified among 530 strains that were previously typed by restriction fragment analysis. Fifteen IS6110 types were identified, but more than half of the strains representing 46 restriction types had the same IS6110 type. Virtually all M. bovis strains as well as strains of M. tuberculosis and Mycobacterium africanum had the same IS1081 type. The results indicate that for M. bovis, IS1081 cannot be used to type strains, IS6110 can be used to distinguish strains into broad groups, but only restriction fragment analysis is sufficiently sensitive for detailed epidemiological studies. An investigation of the host range of IS1081 revealed that, apart from its presence in species of the tuberculosis complex, it is also present in a strain of Mycobacterium xenopi.
牛结核分枝杆菌是牛结核病的病原体，其菌株可通过限制性片段分析相互明确区分。这种DNA指纹识别方法已在新西兰的许多流行病学研究中使用，但该技术存在实际困难，阻碍了其广泛应用。插入元件IS6110被广泛用作区分结核分枝杆菌菌株间限制性片段多态性的DNA探针。该元件以及另一种最近测序的元件IS1081也存在于牛结核分枝杆菌中。我们评估了这两种元件对区分160株牛结核分枝杆菌的有用性。这些菌株大多分离自新西兰，被选为代表之前通过限制性片段分析分型的530株菌株中鉴定出的95种不同类型。鉴定出了15种IS6110类型，但代表46种限制性类型的菌株中，超过一半具有相同的IS6110类型。实际上，所有牛结核分枝杆菌菌株以及结核分枝杆菌和非洲分枝杆菌菌株都具有相同的IS1081类型。结果表明，对于牛结核分枝杆菌，IS1081不能用于菌株分型，IS6110可用于将菌株区分为大致类别，但只有限制性片段分析对详细的流行病学研究足够敏感。对IS1081宿主范围的调查显示，除了存在于结核复合体物种中，它还存在于一株偶发分枝杆菌中。
