Shiba Y, Muneoka Y, Kanno Y
Jpn J Physiol. 1977;27(2):185-93. doi: 10.2170/jjphysiol.27.185.
The transmembrane potential of female rat liver cells in situ was-52.4 mV. This was decreased to -42.9 mV by ethacrynic acid (4 mg/100 g), but not by ouabain (1 mg). DL-Ethionine (25-100 mg) caused a decrease in the membrane potential and tissue ATP content. A high dose ethionine (100 mg) increased tissue Na content and decreased K content. By applying adenine (25 mg) to the animals treated with ethionine (100 mg), the membrane potential, ATP content and K content were increased and the Na content was decreased. The repolarized membrane potential in the animals treated with adenine following the ethionine was again depolarized by the administration of ouabain, but not by the administration of ethacrynic acid. These results suggest that two kinds of active ion transport mechanisms, ethacrynic acid-sensitive and ouabain-sensitive mechanisms, may be involved in maintenance of the membrane potential of rat liver cells.
雌性大鼠原位肝细胞的跨膜电位为-52.4 mV。依他尼酸（4 mg/100 g）可使其降至-42.9 mV，但哇巴因（1 mg）则无此作用。DL-乙硫氨酸（25 - 100 mg）可导致膜电位及组织ATP含量降低。高剂量乙硫氨酸（100 mg）可使组织钠含量增加、钾含量降低。给用乙硫氨酸（100 mg）处理的动物应用腺嘌呤（25 mg）后，膜电位、ATP含量及钾含量增加，钠含量降低。用乙硫氨酸处理后再用腺嘌呤处理的动物中，复极化的膜电位在用哇巴因给药后再次去极化，但依他尼酸给药则无此作用。这些结果表明，大鼠肝细胞膜电位的维持可能涉及两种主动离子转运机制，即依他尼酸敏感机制和哇巴因敏感机制。
