Yanagihara K, Tomono K, Sawai T, Sasaki E, Kakeya H, Yamamoto Y, Ohno H, Ogawa K, Kadota J, Koga H, Kohno S, Hirakata Y, Tashiro T
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Kansenshogaku Zasshi. 1997 Apr;71(4):337-41. doi: 10.11150/kansenshogakuzasshi1970.71.337.
The prognosis of chronic respiratory tract infection with Pseudomonas aeruginosad considered to be poor. However, low-dose and long-term 14 membered macrolide, such as erythromycin or clarithromycin, treatment has been reported as effective clinically in chronic lower respiratory tract disease. There was no report to investigate the effect of macrolide on chronic biofilm related Pseudomonas aeruginosa respiratory tract infection in vivo. In a newly established murine model of chronic Pseudomonas aeruginosa respiratory infection mimicking diffuse panbronchiolitis (DPB), we investigated the effect of erythromcin inhalation on viable bacteria in the lungs. Infection was produced by placement of a plastic tube in the bronchus with inoculating. Pseudomonas aeruginosa suspended in saline was also inoculated in bronchus after intubation of the tube. Viable bacteria were constantly isolated at 10(4) 10(6) cfu-specimen from the lungs for more than 30 days. Treatment with erythromycin inhalation for four days reduced the number of viable bacteria in the lungs with statistically significant. Our result indicated that our new model of chronic respiratory tract infection is simple and provides a useful tool to study the pathogenic process and treatment of such infection. Our results also suggest that the erythromycin inhalation is effective on chronic Pseudomons aeruginosa respiratory infection.
铜绿假单胞菌引起的慢性呼吸道感染预后被认为较差。然而，据报道，低剂量长期使用14元大环内酯类药物，如红霉素或克拉霉素，在临床上对慢性下呼吸道疾病有效。尚无关于大环内酯类药物对慢性生物膜相关铜绿假单胞菌呼吸道感染体内作用的研究报道。在一种新建立的模拟弥漫性泛细支气管炎（DPB）的慢性铜绿假单胞菌呼吸道感染小鼠模型中，我们研究了吸入红霉素对肺内活菌的影响。通过在支气管中放置塑料管并接种来引发感染。在插入管子后，也将悬浮在盐水中的铜绿假单胞菌接种到支气管中。在超过30天的时间里，从肺中持续分离出活菌，每标本10(4) - 10(6) cfu。吸入红霉素治疗四天可使肺内活菌数量减少，具有统计学意义。我们的结果表明，我们新的慢性呼吸道感染模型简单，为研究此类感染的致病过程和治疗提供了有用的工具。我们的结果还表明，吸入红霉素对慢性铜绿假单胞菌呼吸道感染有效。
