Yamamoto S, Hamasaki Y, Ishii E, Ichimaru T, Miyazaki S
Department of Pediatrics, Saga Medical School, Japan.
Ann Allergy Asthma Immunol. 1997 May;78(5):517-23. doi: 10.1016/S1081-1206(10)63241-3.
Cytokines, such as interleukin (IL)-5, produced by T helper type 2 Th2) cells appear to play an important role in the inflammatory processes associated with atopic dermatitis. The roles of cytokines produced by Th1 cells remain controversial.
We examined IL-5 and IL-2 mRNA abundance in and protein production by peripheral blood mononuclear cells (PBMCs) from patients with atopic dermatitis and compared those from controls.
Peripheral blood mononuclear cells were isolated from six children with atopic dermatitis and six control children, and stimulated with both phytohemaggulutinin (PHA) and phorbol 12-myristate 13-acetate (PMA). The abundance of IL-5 and IL-2 mRNA in PBMCs was measured by reverse transcription-polymerase chain reaction analysis. The production of IL-5 and IL-2 by PBMCs was also determined by enzyme-linked immunosorbent assay.
After incubation with PHA and PMA, PBMCs from atopic children showed significantly higher IL-5 mRNA abundance (P < .05) and IL-5 production (P < .01), as well as a lower amount of IL-2 mRNA (P = .056) and IL-2 production (P < .05) than those from healthy controls. The time course of changes in IL-5 mRNA abundance induced by PHA and PMA in PBMCs from atopic children differed markedly from that observed with healthy controls, whereas the time course of changes in IL-2 mRNA abundance were similar between the two groups.
The increased IL-5 and decreased IL-2 production observed with PBMCs from children with atopic dermatitis may underlie the activation of eosinophils and high serum immunoglobulin E concentrations also apparent in such individuals. An imbalance in the number and activity of Th1 and Th2 cells is likely to be responsible for the abnormal pattern of cytokine production in atopic dermatitis.
2型辅助性T细胞（Th2）产生的细胞因子，如白细胞介素（IL）-5，似乎在与特应性皮炎相关的炎症过程中起重要作用。Th1细胞产生的细胞因子的作用仍存在争议。
我们检测了特应性皮炎患者外周血单个核细胞（PBMC）中IL-5和IL-2 mRNA丰度及其蛋白产生情况，并与对照组进行比较。
从6名特应性皮炎儿童和6名对照儿童中分离外周血单个核细胞，并用植物血凝素（PHA）和佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）进行刺激。通过逆转录-聚合酶链反应分析测量PBMC中IL-5和IL-2 mRNA的丰度。PBMC产生的IL-5和IL-2也通过酶联免疫吸附测定法进行测定。
与健康对照组相比，特应性儿童的PBMC在与PHA和PMA孵育后，IL-5 mRNA丰度显著更高（P <.05），IL-5产生量显著更高（P <.01），而IL-2 mRNA量更低（P =.056），IL-2产生量更低（P <.05）。PHA和PMA诱导的特应性儿童PBMC中IL-5 mRNA丰度变化的时间进程与健康对照组明显不同，而两组之间IL-2 mRNA丰度变化的时间进程相似。
特应性皮炎儿童的PBMC中观察到的IL-5增加和IL-2产生减少可能是这些个体中嗜酸性粒细胞活化和高血清免疫球蛋白E浓度的基础。Th1和Th2细胞数量和活性的失衡可能是特应性皮炎中细胞因子产生异常模式的原因。
