通过使用能区分鸟分枝杆菌和胞内分枝杆菌的IS901检测引物，研究IS901在猪源鸟分枝杆菌复合群菌株中的分布情况。
Distribution of IS901 in strains of Mycobacterium avium complex from swine by using IS901-detecting primers that discriminate between M. avium and Mycobacterium intracellulare.
作者信息
Nishimori K, Eguchi M, Nakaoka Y, Onodera Y, Ito T, Tanaka K
机构信息
Hokkaido Branch, National Institute of Animal Health, Sapporo, Japan.
出版信息
J Clin Microbiol. 1995 Aug;33(8):2102-6. doi: 10.1128/jcm.33.8.2102-2106.1995.
Abstract
The presence of the mycobacterial insertion sequence IS901 was studied by PCR with reference strains of Mycobacterium avium complex; 122 veterinary strains of mycobacteria, mainly M. avium complex, isolated from swine; and 15 clinical strains. Four kinds of DNA extraction methods for PCR were compared. Use of the commercial extraction matrix allowed for the faster and easier preparation of PCR-amplifiable DNA than use of NaOH heating extraction or sodium dodecyl sulfate extraction of pretreated mycobacteria. It also provided more effective protection than boiling extraction against the destruction of DNA. Four reference strains of serovars 1 to 3 possessed IS901. Nine reference strains of serovars 1, 4 to 6, 8 to 11, and 21 possessed only IS901 insertion sites. A novel PCR product was found in the other reference strains of serovars 7, 12 to 17, 19, and 20 and two clinical strains of serovar 15. It is suggested that the primers that amplified the insertion portion of IS901 divided the M. avium complex into M. avium, Mycobacterium intracellulare, and other mycobacteria. None of the 110 strains of M. avium complex isolated from swine possessed IS901. It is suggested that the absence of IS901 might be characteristic of swine-derived strains of M. avium complex.
摘要
采用针对鸟分枝杆菌复合群参考菌株的聚合酶链反应（PCR），研究分枝杆菌插入序列IS901的存在情况；122株主要从猪分离得到的兽用分枝杆菌菌株，主要为鸟分枝杆菌复合群；以及15株临床菌株。比较了用于PCR的四种DNA提取方法。与使用氢氧化钠加热提取或对预处理分枝杆菌进行十二烷基硫酸钠提取相比，使用商业提取基质能更快、更轻松地制备可用于PCR扩增的DNA。与煮沸提取相比，它还能更有效地保护DNA不被破坏。血清型1至3的四株参考菌株含有IS901。血清型1、4至6、8至11和21的九株参考菌株仅含有IS901插入位点。在血清型7、12至17、19和20的其他参考菌株以及血清型15的两株临床菌株中发现了一种新的PCR产物。提示扩增IS901插入部分的引物可将鸟分枝杆菌复合群分为鸟分枝杆菌、胞内分枝杆菌和其他分枝杆菌。从猪分离得到的110株鸟分枝杆菌复合群菌株均不含有IS901。提示IS901缺失可能是猪源鸟分枝杆菌复合群菌株的特征。
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