Bellini R, Casali B, Carrieri M, Zambonelli C, Rivasi P, Rivasi F
Centro Agricoltura Ambiente, Crevalcore, Italy.
APMIS. 1997 Apr;105(4):299-302. doi: 10.1111/j.1699-0463.1997.tb00572.x.
In laboratory experiments, Aedes albopictus females were fed hepatitis C virus (HCV)-infected human blood with a unit-feeding apparatus. Pools of engorged females were tested at various time intervals after their blood meal for the presence of HCV RNA using two methods in parallel: reverse transcription-nested polymerase chain reaction (PCR) and PCR with the Amplicor HCV system. Comparable results were obtained with both methods. Mosquitoes were found to be positive only immediately and 24 h after feeding. Moreover, female progeny produced negative results. Our results indicate that the Ae.albopictus strain used is incompetent as a vector of HCV.
在实验室实验中，用单位喂食装置给白纹伊蚊雌蚊喂食感染丙型肝炎病毒（HCV）的人血。在饱血雌蚊进食后，在不同时间间隔用两种方法并行检测HCV RNA的存在：逆转录巢式聚合酶链反应（PCR）和使用Amplicor HCV系统的PCR。两种方法得到了可比的结果。仅在喂食后即刻和24小时发现蚊子呈阳性。此外，雌性后代检测结果为阴性。我们的结果表明，所用的白纹伊蚊品系作为HCV的载体是无能力的。
