Silverman A L, McCray D G, Gordon S C, Morgan W T, Walker E D
Department of Medicine, William Beaumont Hospital, Royal Oak, MI 48073, USA.
J Med Entomol. 1996 May;33(3):398-401. doi: 10.1093/jmedent/33.3.398.
In 3 laboratory experiments, mosquitoes were fed hepatitis C virus (HCV)-RNA positive blood by using membrane feeders, separated into head, thorax, and abdomen, and tested by a reverse transcriptase polymerase chain reaction for HCV-RNA. HCV did not replicate or disseminate in mosquitoes that had ingested blood from patients that were HCV-viremic positive. When yellow fever mosquitoes, Aedes aegypti (L.), were held for 1, 3, 7, 14, and 21 d after feeding, HCV-RNA was detected in the abdomens of 5/5 mosquitoes at 1 d after feeding; remaining tissues were negative with the exception of a single positive head at 7 d. In agreement, HCV-RNA was detected in Asian tiger mosquito, Aedes albopictus Skuse, and Anopheles stephensi Liston abdomens at 1 d, but not 3 d after feeding no HCV-RNA was detected in heads or thoraces. In addition, HCV-RNA was detected in heads of Ae. aegypti at 10 and 20 min, but not at 30 min, after feeding. The latter results raise the possibility of HCV contamination of mouthparts and, theoretically, mechanical transmission of this virus.
在3项实验室实验中，使用膜饲器给蚊子喂食丙型肝炎病毒（HCV）-RNA阳性血液，将蚊子分为头部、胸部和腹部，并通过逆转录聚合酶链反应检测HCV-RNA。HCV在摄入HCV病毒血症阳性患者血液的蚊子中未复制或传播。当埃及伊蚊在进食后分别饲养1、3、7、14和21天时，在进食后1天，5只蚊子中有5只的腹部检测到HCV-RNA；除了在7天时1个头部呈阳性外，其余组织均为阴性。同样，在亚洲虎蚊白纹伊蚊和斯氏按蚊进食后1天，在其腹部检测到HCV-RNA，但在进食后3天，在头部或胸部未检测到HCV-RNA。此外，在埃及伊蚊进食后10分钟和20分钟时，在其头部检测到HCV-RNA，但在30分钟时未检测到。后一结果增加了口器被HCV污染的可能性，从理论上讲，也增加了该病毒机械传播的可能性。
