Ballmer-Weber B K, Braathen L R, Brand C U
Department of Dermatology, University of Berne Inselspital, Switzerland.
Arch Dermatol Res. 1997 Apr;289(5):251-5. doi: 10.1007/s004030050188.
The expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin is important for the regulation of the leucocyte traffic into and in inflammatory dermatoses. ICAM-1, VCAM-1 and E-selectin were initially identified as cell-surface proteins, but recent evidence suggests that they also exist in a soluble form. The collection of human afferent lymph exclusively deriving from a selected skin area allows insight into local pathomechanisms as well as signal transmission in skin disorders. In the present study we measured the concentrations of the soluble adhesion molecules (sAM) sICAM-1, sVCAM-1 and sE-selectin in human skin lymph derived from normal untreated skin, irritant contact dermatitis (CD) and the induction and elicitation phases of allergic CD. The strong elicitation reactions of allergic CD produced an increase in sAM output to about three times the baseline values but in the weaker irritant CD we observed no increase at all. In the induction phase of allergic CD the concentrations during the first 9 days of the experiment remained unchanged, as in the lymph derived from normal untreated skin, but were slightly increased thereafter. To our knowledge, no in vivo data exist on the local involvement of sAM in irritant and allergic CD in humans. Our results provide evidence of increased concentrations of sAM mainly in strong allergic CD.
细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和E-选择素的表达对于调节白细胞进入炎症性皮肤病以及在其中的流动至关重要。ICAM-1、VCAM-1和E-选择素最初被鉴定为细胞表面蛋白，但最近的证据表明它们也以可溶性形式存在。专门从选定皮肤区域采集的人体传入淋巴液有助于深入了解皮肤疾病的局部发病机制以及信号传递。在本研究中，我们测量了来自正常未处理皮肤、刺激性接触性皮炎（CD）以及过敏性CD诱导期和激发期的人体皮肤淋巴液中可溶性黏附分子（sAM）sICAM-1、sVCAM-1和sE-选择素的浓度。过敏性CD的强烈激发反应使sAM输出增加至基线值的约三倍，但在较弱的刺激性CD中我们根本未观察到增加。在过敏性CD的诱导期，实验前9天的浓度与来自正常未处理皮肤的淋巴液一样保持不变，但此后略有增加。据我们所知，尚无关于sAM在人类刺激性和过敏性CD中局部参与情况的体内数据。我们的结果提供了证据，表明sAM浓度增加主要见于强烈的过敏性CD。
