Hannestad U, Lundblad A
Department of Clinical Chemistry, University Hospital, Linköping, Sweden.
Clin Chem. 1997 May;43(5):794-800.
An accurate and precise method to determine glucose concentration in whole blood is presented. The method, based on isotope dilution gas chromatography-mass spectrometry (ID GC-MS), was developed to be used as a Reference Method for determining glucose concentration in capillary or venous whole blood. Blood samples and standards are pipetted manually with "microcap" micropipettes, which makes it possible to collect samples even at the patient's bedside. Glucose is quantified as its aldononitrile pentaacetate. [13C6]Glucose is used as an internal standard. Assay of Seronorm and Pathonorm L and H controls by ID GC-MS gave within-run CVs of 0.66%, 0.96%, and 0.92%, respectively. For whole blood with glucose concentrations in the low, normal, and high ranges, the within-run CVs were 1.27%, 0.91%, and 0.78%, respectively. The between-run CV for glucose calculated from 36 separate single analyses of Seronorm was 1.44%. In an accuracy assessment test of the HemoCue blood glucose analyzer, 140 capillary blood samples were measured in parallel after split-sampling. For all samples the HemoCue analyzer results had a mean bias of +2.0% compared with the ID GC-MS results.
本文介绍了一种准确且精密的测定全血中葡萄糖浓度的方法。该方法基于同位素稀释气相色谱-质谱联用技术（ID GC-MS）开发，旨在用作测定毛细血管或静脉全血中葡萄糖浓度的参考方法。血液样本和标准品使用“微量帽”微量移液器手动移液，这使得即使在患者床边也能采集样本。葡萄糖以其戊五乙酸醛腈形式进行定量。[13C6]葡萄糖用作内标。通过ID GC-MS对血清标准品和病理标准品L及H进行检测，批内变异系数（CV）分别为0.66%、0.96%和0.92%。对于葡萄糖浓度处于低、正常和高范围的全血，批内CV分别为1.27%、0.91%和0.78%。通过对血清标准品进行36次单独的单次分析计算得出的葡萄糖批间CV为1.44%。在对HemoCue血糖仪的准确性评估测试中，对140份毛细血管血样本进行了分样后平行测量。与ID GC-MS结果相比，所有样本的HemoCue血糖仪结果平均偏差为+2.0%。
