Maleewong W, Intapan P M, Ieamviteevanich K, Wongkham C, Morakote N
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
J Helminthol. 1997 Mar;71(1):69-71. doi: 10.1017/s0022149x00000821.
Early third-stage larvae of Gnathostoma spinigerum were cultured in RPMI-1640 supplemented with 25mM NaHCO3,100 units/ml penicillin G, 100 microg/ml of streptomycin, 5 microg/ml of amphotericin B and 10% foetal calf serum at 37 degrees C under 5% CO2 in air for 60 days. After 3 days of cultivation, the larvae moulted. Body sizes increased from 0.49 +/- 0.09 x 0.07 +/- 0.01 mm in length and width to 4.08 +/- 0.48 x 0.32 +/- 0.04 mm after 60 days of cultivation. The maximum body length and width of these larvae were 4.94 mm and 0. 35 mm, respectively. The survival rate (67.5 %) of the worms was observed at the end of cultivation. The addition of foetal calf serum was found to be essential for growth and development.
将棘颚口线虫的早期三期幼虫置于补充有25mM碳酸氢钠、100单位/毫升青霉素G、100微克/毫升链霉素、5微克/毫升两性霉素B和10%胎牛血清的RPMI-1640培养基中，于37℃、5%二氧化碳的空气环境下培养60天。培养3天后，幼虫蜕皮。培养60天后，虫体大小从长度0.49±0.09毫米、宽度0.07±0.01毫米增长至长度4.08±0.48毫米、宽度0.32±0.04毫米。这些幼虫的最大体长和体宽分别为4.94毫米和0.35毫米。培养结束时观察到虫体的存活率为（67.5%）。发现添加胎牛血清对其生长发育至关重要。
