Maleewong W, Intapan P M, Khempila J, Wongwajana S, Wongkham C, Morakote N
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.
J Parasitol. 1995 Oct;81(5):800-3.
Advanced third-stage larvae of Gnathostoma spinigerum were cultured in RPMI-1640, with various supplements at 37 C under 5% CO2 in air for 300 days. The most suitable medium supplement for worm development was 10% fetal calf serum, 1% dog serum, and 0.25% dog hemolysate. After approximately 180 days of cultivation, some larvae molted to the fourth stage as distinguished by 8 transverse rows of cephalic hooklets and well differentiated sex organs. The maximum body length and width of these larvae were 18.6 mm and 1.1 mm, respectively. Six of 50 larvae (12%) developed to the fourth stage, with a 32% survival rate at the end of cultivation. Although the highest survival rate (70%) of the worms was observed in the medium supplemented with 25 mM NaHCO3, only 4% developed into fourth stage larvae. The addition of fetal calf serum, dog serum, and dog hemolysate was essential for growth and development.
棘颚口线虫的晚期三期幼虫在添加了各种补充剂的RPMI-1640培养基中，于37℃、5%二氧化碳的空气环境下培养300天。对虫体发育而言，最合适的培养基补充剂是10%胎牛血清、1%犬血清和0.25%犬溶血产物。培养约180天后，部分幼虫蜕变为四期，其特征为有8排横向排列的头部小钩且性器官分化良好。这些幼虫的最大体长和体宽分别为18.6毫米和1.1毫米。50条幼虫中有6条（12%）发育至四期，培养结束时存活率为32%。尽管在添加25 mM碳酸氢钠的培养基中观察到虫体的最高存活率（70%），但只有4%发育为四期幼虫。添加胎牛血清、犬血清和犬溶血产物对其生长发育至关重要。
