Croes K, Van Veldhoven P P, Mannaerts G P, Casteels M
Katholieke Universiteit Leuven, Afdeling Farmacologie, Campus Gasthuisberg, Belgium.
FEBS Lett. 1997 Apr 28;407(2):197-200. doi: 10.1016/s0014-5793(97)00343-8.
alpha-Oxidation of 3-methyl-substituted fatty acids was studied in purified rat liver peroxisomes. The experiments revealed that formyl-CoA is formed during the alpha-oxidation process. The amount of formyl-CoA found constituted 2-5% of the amount of formate formed. Under the conditions used, no activation of exogenously added formate occurred in purified peroxisomes, whereas 95.5% of added synthetic formyl-CoA was converted to formate. These data indicate that during alpha-oxidation first formyl-CoA is formed, which is then hydrolysed to formate.
在纯化的大鼠肝脏过氧化物酶体中研究了3-甲基取代脂肪酸的α-氧化。实验表明，在α-氧化过程中会形成甲酰辅酶A。所发现的甲酰辅酶A的量占所形成甲酸盐量的2-5%。在所使用的条件下，纯化的过氧化物酶体中未发生外源添加甲酸盐的活化，而添加的合成甲酰辅酶A有95.5%转化为甲酸盐。这些数据表明，在α-氧化过程中首先形成甲酰辅酶A，然后其被水解为甲酸盐。
