Kamat A S, Khare S, Doctor T, Nair P M
Food Technology Division, Bhabha Atomic Research Centre, Trombay, India.
Int J Food Microbiol. 1997 Apr 29;36(1):69-76. doi: 10.1016/s0168-1605(96)01249-4.
gamma-Radiation response of Y. enterocolitica 5692 and 152 was studied at 0 degrees C and at -40 degrees C in phosphate buffer (pH 7.00) as well as in 10% raw meat/salami homogenate. The strains investigated did not differ in their response and were found to be sensitive to gamma-radiation but exhibited a tailing phenomenon in the survival curve. The D10 in homogenate was 0.25 kGy at 0 degrees C. This response was not affected at -40 degrees C. Storage studies of packs, inoculated artificially with heavy inoculum of Y. enterocolitica (10(6) cfu/g) showed that while samples of salami and cooked ham could be decontaminated at doses of 4 and 3 kGy respectively; cells could not be eliminated from raw pork meat even at the higher dose of 6 kGy. The role of different treatments given prior to irradiation for revival of Y. enterocolitica after irradiation storage was studied. The dose of 1 kGy at -40 degrees C was efficient in eradicating low numbers (< 10(3)) of naturally occurring of Y. enterocolitica from raw pork meat without any revival during storage at refrigeration temperature.
在0℃和-40℃条件下，于磷酸盐缓冲液（pH 7.00）以及10%生肉/萨拉米香肠匀浆中研究了小肠结肠炎耶尔森氏菌5692和152的γ辐射反应。所研究的菌株在反应上没有差异，发现它们对γ辐射敏感，但在存活曲线上表现出拖尾现象。在0℃时，匀浆中的D10为0.25千戈瑞。在-40℃时，这种反应不受影响。对接种了高浓度小肠结肠炎耶尔森氏菌（10⁶ cfu/g）的包装进行的储存研究表明，虽然萨拉米香肠和熟火腿样品分别可以用4千戈瑞和3千戈瑞的剂量进行去污；但即使在6千戈瑞的较高剂量下，生猪肉中的细胞也无法消除。研究了辐照前进行不同处理对辐照储存后小肠结肠炎耶尔森氏菌复苏的作用。在-40℃时，1千戈瑞的剂量能有效消除生猪肉中少量（<10³）自然存在的小肠结肠炎耶尔森氏菌，在冷藏温度下储存期间不会有任何复苏情况。
