Shibayama H, Machii T, Tokumine Y, Nishimori Y, Nojima J, Inoue R, Kanamaru A, Tagawa S, Kitani T
Department of Hematology, Osaka University Medical School, Japan.
Leuk Lymphoma. 1997 Apr;25(3-4):373-80. doi: 10.3109/10428199709114176.
A cell line, JHC-2, was established from the peripheral blood of a patient with hairy cell leukemia (HCL)-Japanese variant. The JHC-2 cells have cytologic features similar to those of the original tumor cells. They displayed hairy cytoplasmic projections by phase contrast and scanning electron microscopy. The tartrate-resistant acid phosphatase reaction was weakly positive. The immunophenotype of the JHC-2 cells was CD5-, CD10-, CD11c+/-, CD19+, CD21+, CD23+, CD24-, CD25+/-, CD38- and FMC-7+. The expression of surface immunoglobulin (IgG, kappa) and the configuration of Ig gene rearrangements in the JHC-2 cells were identical to those in the original leukemic cells, and the JHC-2 cells displayed trisomy 9 on cytogenetic examination. Southern blot analysis for the Epstein-Barr virus (EBV) genome showed that the JHC-2 cells contained the EBV genome, although the freshly isolated leukemic cells did not. These results indicate that the JHC-2 cell line is an EBV spontaneously transformed B cell line originating from HCL cells.
JHC - 2细胞系是从一名日本变异型毛细胞白血病（HCL）患者的外周血中建立的。JHC - 2细胞具有与原始肿瘤细胞相似的细胞学特征。通过相差显微镜和扫描电子显微镜观察，它们呈现出毛状细胞质突起。抗酒石酸酸性磷酸酶反应呈弱阳性。JHC - 2细胞的免疫表型为CD5 -、CD10 -、CD11c + / -、CD19 +、CD21 +、CD23 +、CD24 -、CD25 + / -、CD38 -和FMC - 7 +。JHC - 2细胞表面免疫球蛋白（IgG，κ）的表达和Ig基因重排的构型与原始白血病细胞相同，并且在细胞遗传学检查中JHC - 2细胞显示9号染色体三体性。对爱泼斯坦 - 巴尔病毒（EBV）基因组的Southern印迹分析表明，JHC - 2细胞含有EBV基因组，尽管新鲜分离的白血病细胞不含该基因组。这些结果表明JHC - 2细胞系是源自HCL细胞的EBV自发转化B细胞系。
