Tokioka T, Shimamoto Y, Nagumo F, Tadano J, Yamaguchi M
Department of Internal Medicine, Saga Medical School, Japan.
Acta Haematol. 1994;92(1):8-13. doi: 10.1159/000204129.
A hairy-cell leukaemia (HCL) cell line, HCL-O, was established from the peripheral blood of a 62-year-old Japanese patient with a unique variant of HCL strongly expressing CD21, the receptor for the Epstein-Barr virus (EBV). The HCL-O cells expressed antigens similar and dissimilar to those expressed with the original hairy cells. The HCL-O cells were more mature than the original cells in their degree of B-cell differentiation, as indicated by a decrease of CD19 and surface immunoglobulin (sIg) expression together with the appearance of CD38 and cytoplasmic Ig (cIg). In addition, the cells expressed CD11c recognized by Leu-M5, a monoclonal antibody usually positive for HCL. Their karyotype and Ig gene rearrangement pattern were identical to those of the original cells. The EBV genome was detected in the HCL-O cells but not in the original cells. The HCL-O cells spontaneously produced a large quantity of interleukin-6 (IL-6) in the conditioned medium, whereas IL-6 serum level was not so high. These findings indicate that the HCL-O cell line is derived from the leukaemic hairy cells and possibly, in vitro EBV infection took place easily in the original hairy cells through their CD21, resulting in subsequent immortalization. IL-6 production by HCL-O cells may be induced or enhanced by EBV, and the secreted IL-6 might play a role in their own growth or differentiation.
一种毛细胞白血病（HCL）细胞系HCL-O，是从一名62岁日本HCL独特变异型患者的外周血中建立的，该患者强烈表达EB病毒（EBV）受体CD21。HCL-O细胞表达的抗原与原始毛细胞表达的抗原有相似和不同之处。HCL-O细胞在B细胞分化程度上比原始细胞更成熟，表现为CD19和表面免疫球蛋白（sIg）表达降低，同时出现CD38和细胞质免疫球蛋白（cIg）。此外，这些细胞表达Leu-M5识别的CD11c，Leu-M5是一种通常对HCL呈阳性的单克隆抗体。它们的核型和Ig基因重排模式与原始细胞相同。在HCL-O细胞中检测到了EBV基因组，而原始细胞中未检测到。HCL-O细胞在条件培养基中自发产生大量白细胞介素-6（IL-6），而IL-6血清水平并不高。这些发现表明，HCL-O细胞系源自白血病毛细胞，并且可能原始毛细胞通过其CD21在体外容易发生EBV感染，从而导致随后的永生化。HCL-O细胞产生IL-6可能由EBV诱导或增强，分泌的IL-6可能在其自身生长或分化中起作用。
