Fang J, Kushida S, Feng R, Tanaka M, Kikukawa H, Kawamura T, Uchida K, Miwa M
Department of Biochemistry and Molecular Oncology, University of Tsukuba, Ibaraki, Japan.
Biochem Biophys Res Commun. 1997 Apr 28;233(3):792-5. doi: 10.1006/bbrc.1997.6557.
Human T cell leukemia virus type 1 (HTLV-1) provirus integration was investigated in mice inoculated with MT-2 cells, a HTLV-1 producing human T-cell line. From spleen DNA derived from a MT-2 cell-injected mouse, we cloned a HTLV-1 integration site by ligation-mediated PCR. The nucleotide sequence showed that HTLV-1 provirus was integrated into an intron of the mouse transforming growth factor-alpha gene in the reverse orientation. This result provides for the first time molecular evidence that mice can be infected with HTLV-1.
在接种了MT - 2细胞（一种产生人类T细胞白血病病毒1型（HTLV - 1）的人类T细胞系）的小鼠中研究了HTLV - 1前病毒整合情况。从一只注射了MT - 2细胞的小鼠的脾脏DNA中，我们通过连接介导的PCR克隆了一个HTLV - 1整合位点。核苷酸序列显示HTLV - 1前病毒以反向方向整合到小鼠转化生长因子α基因的一个内含子中。这一结果首次提供了小鼠可被HTLV - 1感染的分子证据。
