Susloparov M A, Krendel'shchikov A V, Bakhtina M M, Bedristov A I, Babkin I V, Gutorov V V, Eroshkin A M, Mamaeva O A
Mol Gen Mikrobiol Virusol. 1996 Apr-Jun(2):32-9.
MT-4 cell line is a continuous strain of human T lymphocytes expressing defective noninfective subviral HTLV-1 particles. A fragment of sequence encoding the p24 protein and gene for envelope protein (env) have been obtained from genomic DNA of this culture by polymerase chain reaction. Both HTLV-1 fragments were cloned in bacterial vectors, and the nucleotide sequence of these regions was determined. The cloned DNA fragment encoding the p24 has only four point nucleotide exchanges. Analysis of the env gene structure revealed that the sequence had several amino acid exchanges and two deletions (13 bp and 70 bp).
MT - 4细胞系是表达有缺陷的非感染性亚病毒HTLV - 1颗粒的人T淋巴细胞连续株。通过聚合酶链反应从该培养物的基因组DNA中获得了编码p24蛋白的序列片段和包膜蛋白（env）基因。这两个HTLV - 1片段都克隆到细菌载体中，并测定了这些区域的核苷酸序列。编码p24的克隆DNA片段只有四个点核苷酸交换。对env基因结构的分析表明，该序列有几个氨基酸交换和两个缺失（13bp和70bp）。
