Spicer A P, Seldin M F, Olsen A S, Brown N, Wells D E, Doggett N A, Itano N, Kimata K, Inazawa J, McDonald J A
Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Arizona 85259, USA.
Genomics. 1997 May 1;41(3):493-7. doi: 10.1006/geno.1997.4696.
We have recently identified a new vertebrate gene family encoding putative hyaluronan (HA) synthases. Three highly conserved related genes have been identified, designated HAS1, HAS2, and HAS3 in humans and Has1, Has2, and Has3 in the mouse. All three genes encode predicted plasma membrane proteins with multiple transmembrane domains and approximately 25% amino acid sequence identity to the Streptococcus pyogenes HA synthase, HasA. Furthermore, expression of any one HAS gene in transfected mammalian cells leads to high levels of HA biosynthesis. We now report the chromosomal localization of the three HAS genes in human and in mouse. The genes localized to three different positions within both the human and the mouse genomes. HAS1 was localized to the human chromosome 19q13.3-q13.4 boundary and Has1 to mouse Chr 17.HAS2 was localized to human chromosome 8q24.12 and Has2 to mouse Chr 15. HAS3 was localized to human chromosome 16q22.1 and Has3 to mouse Chr 8. The map position for HAS1 reinforces the recently reported relationship between a small region of human chromosome 19q and proximal mouse chromosome 17. HAS2 mapped outside the predicted critical region delineated for the Langer-Giedion syndrome and can thus be excluded as a candidate gene for this genetic syndrome.
我们最近鉴定出一个新的脊椎动物基因家族，其编码假定的透明质酸（HA）合酶。已鉴定出三个高度保守的相关基因，在人类中命名为HAS1、HAS2和HAS3，在小鼠中命名为Has1、Has2和Has3。所有这三个基因都编码预测的具有多个跨膜结构域的质膜蛋白，并且与化脓性链球菌HA合酶HasA具有约25%的氨基酸序列同一性。此外，任何一个HAS基因在转染的哺乳动物细胞中的表达都会导致高水平的HA生物合成。我们现在报告这三个HAS基因在人类和小鼠中的染色体定位。这些基因定位于人类和小鼠基因组内的三个不同位置。HAS1定位于人类染色体19q13.3 - q13.4边界，Has1定位于小鼠第17号染色体。HAS2定位于人类染色体8q24.12，Has2定位于小鼠第15号染色体。HAS3定位于人类染色体16q22.1，Has3定位于小鼠第8号染色体。HAS1的图谱位置加强了最近报道的人类染色体19q的一个小区域与小鼠近端染色体17之间的关系。HAS2定位于为朗格-吉迪恩综合征划定的预测关键区域之外，因此可以排除作为该遗传综合征候选基因的可能性。
