Tien Janet Y L, Spicer Andrew P
Department of Cell Biology and Human Anatomy, University of California, School of Medicine, 1 Shields Avenue, Davis, CA 95616, USA.
Dev Dyn. 2005 May;233(1):130-41. doi: 10.1002/dvdy.20328.
We have used in situ hybridization to study the expression of the vertebrate hyaluronan synthase (Has) gene family members, designated Has1, Has2, and Has3, during mouse development. At embryonic day (E) 7.5, Has1 and Has2 are expressed throughout the gastrulating embryo. After E8.5, Has1 expression disappears, but Has2 continues to be strongly, albeit transiently, expressed in numerous tissues, including the branchial arches and craniofacial structures such as the palatal shelves and lens pit. Has2 is also expressed during cardiac, skeletal, and tail development. Has3 transcripts are first detected at E10.5 in the maxillary and mandibular components of the first branchial arch. Notably, Has3 expression in the developing teeth, vibrissae hair follicles, nasal cavity, and inner ear complements the expression pattern of Has2. Our results indicate that, whereas Has2 is exclusively expressed in some tissues, its expression pattern overlaps and/or complements that of Has1 and Has3 in others.
我们利用原位杂交技术研究了脊椎动物透明质酸合酶（Has）基因家族成员Has1、Has2和Has3在小鼠发育过程中的表达情况。在胚胎第7.5天（E7.5），Has1和Has2在整个原肠胚形成期的胚胎中表达。在E8.5之后，Has1的表达消失，但Has2在包括鳃弓和腭架及晶状体窝等颅面结构在内的许多组织中持续强烈表达，尽管是短暂的。Has2在心脏、骨骼和尾部发育过程中也有表达。Has3转录本最早在E10.5时在第一鳃弓的上颌和下颌部分被检测到。值得注意的是，Has3在发育中的牙齿、触须毛囊、鼻腔和内耳中的表达与Has2的表达模式互补。我们的结果表明，虽然Has2仅在某些组织中表达，但其表达模式在其他组织中与Has1和Has3的表达模式重叠和/或互补。
