Pennings S
Department of Biochemistry, University of Edinburgh, Scotland.
Methods. 1997 May;12(1):20-7. doi: 10.1006/meth.1997.0443.
Nucleoprotein gel electrophoresis, as well as its use in chromatin research, is reviewed from its early application in the characterization of native nucleosome composition to current uses in analyzing transcription factor-nucleosome complexes and in visualizing multiple nucleosome positioning. Despite our incomplete understanding of the principles behind the separation of particles in native polyacrylamide, gels, powerful new applications of the method have emerged in recent years. This offers the potential for novel experimental strategies, such as those that have led to the discovery of nucleosome mobility.
本文综述了核蛋白凝胶电泳及其在染色质研究中的应用，内容涵盖从其早期用于天然核小体组成特征分析到当前用于分析转录因子 - 核小体复合物以及可视化多个核小体定位的应用。尽管我们对天然聚丙烯酰胺凝胶中颗粒分离背后的原理理解并不完全，但近年来该方法出现了强大的新应用。这为新的实验策略提供了潜力，例如那些导致发现核小体迁移率的策略。
