Henderson Z
Department of Physiology, University of Leeds, U.K.
Neuroscience. 1997 Jun;78(4):943-55. doi: 10.1016/s0306-4522(96)00636-7.
Previous studies have shown that the striatum provides synaptic inputs to the globus pallidus and entopeduncular nucleus in which GABA is co-localized with the peptides enkephalin and substance P. The aim of this study in the rat was to determine whether the striatal projections also make synaptic contact with the cholinergic neurons of the nucleus basalis, which lie near to the pallidal areas in the rat brain. The anterograde tracer biocytin was injected into different parts of the striatum, and brain sections were stained for biocytin and choline acetyltransferase immunoreactivity by using a dual colour method. Terminals labelled with biocytin by anterograde transport and which made synaptic contact with choline acetyltransferase-positive soma and dendrites were identified by light-electron microscopic correlation methods. In the cases where the biocytin injections had been made in the dorsal or lateral striatum, biocytin-labelled terminals made synaptic contact with cholinergic cells in the region between the main termination zones in the globus pallidus and the entopeduncular nucleus. In the cases where the injections had been made in the ventromedial and posterior striatum, there was greater overlap between choline acetyltransferase-positive structures and biocytin-labelled terminals in the main termination zones in the globus pallidus or entopeduncular nucleus, but relatively few of these terminals made synaptic contacts on to the cholinergic neurons. The results therefore indicate that the cholinergic nucleus basalis cells receive a relatively sparse synaptic input from all parts of the striatum. It has recently been shown that the cholinergic cells of the nucleus basalis selectively express high levels of substance P and opioid receptor messenger RNAs, while the non-cholinergic pallidal cells have much higher levels of GABA(A) receptor subunit messenger RNAs. It is concluded that the cholinergic neurons of the nucleus basalis in the rat may be selectively responsive to the peptidergic components of the striatal outputs, and that they are most likely to be influenced by both the limbic and sensorimotor parts of the striatum.
先前的研究表明，纹状体向苍白球和内苍白球核提供突触输入，其中γ-氨基丁酸（GABA）与脑啡肽和P物质肽共定位。本研究在大鼠中的目的是确定纹状体投射是否也与基底核的胆碱能神经元形成突触联系，这些胆碱能神经元位于大鼠脑内的苍白球区域附近。将顺行示踪剂生物胞素注射到纹状体的不同部位，并使用双色法对脑切片进行生物胞素和胆碱乙酰转移酶免疫反应性染色。通过光镜-电镜关联方法鉴定经顺行运输标记有生物胞素且与胆碱乙酰转移酶阳性的胞体和树突形成突触联系的终末。在生物胞素注射到背侧或外侧纹状体的情况下，生物胞素标记的终末与苍白球和内苍白球核主要终末区之间区域的胆碱能细胞形成突触联系。在注射到腹内侧和后纹状体的情况下，胆碱乙酰转移酶阳性结构与苍白球或内苍白球核主要终末区的生物胞素标记终末之间有更大的重叠，但这些终末中相对较少与胆碱能神经元形成突触联系。因此，结果表明基底核的胆碱能细胞从纹状体的所有部位接受相对稀疏的突触输入。最近的研究表明，基底核的胆碱能细胞选择性地高水平表达P物质和阿片受体信使核糖核酸，而非胆碱能苍白球细胞具有更高水平的γ-氨基丁酸A（GABAA）受体亚基信使核糖核酸。得出的结论是，大鼠基底核的胆碱能神经元可能对纹状体输出的肽能成分有选择性反应，并且它们最有可能受到纹状体边缘和感觉运动部分的影响。
