Illana M, Prada A, Verástegui C, González Macías M C, Fernández-Trujillo F J
Department of Morphological Sciences, Faculty of Medicine, University of Cádiz (UCA), Spain.
Eur J Histochem. 1997;41(1):41-6.
We used a group of lectins (Con-A, WGA, SBA, DBA, RCA-1, UEA-1), enzymes (neuraminidase digestion) and conventional histochemical techniques (periodic acid-Schiff reaction and reduction-saponification-Schiff reaction) in order to detect the presence of glycoproteins rich in sialic and neuraminic acids in the human eccrine sweat glands. Using both identification systems, our results showed an abundant secretion, rich in C8Oxi-acylated sialic acid.
我们使用了一组凝集素(刀豆球蛋白A、小麦胚凝集素、大豆凝集素、荆豆凝集素、蓖麻凝集素-1、荆豆凝集素-1)、酶(神经氨酸酶消化)和传统组织化学技术(过碘酸-希夫反应和还原-皂化-希夫反应),以检测人外泌汗腺中富含唾液酸和神经氨酸的糖蛋白的存在。使用这两种鉴定系统,我们的结果显示有大量分泌,富含C8Oxi-酰化唾液酸。