Rojo M C, Blánquez M J, González M E
Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, U.C.M., Madrid, Spain.
J Anat. 1996 Dec;189 ( Pt 3)(Pt 3):609-21.
A histochemical study of the branchial area of brown trout embryos from 35 to 71 d of incubation is reported. A battery of 6 different horseradish peroxidase-labelled lectins, the PAS reaction and Alcian blue staining were used to study the distribution of carbohydrate residues in glycoconjugates along the pharyngeal and branchial epithelia. Con A and WGA reacted at every site of the branchial region thus showing the ubiquitous presence of alpha-D-mannose and N-acetyl-D-glucosamine. WGA, DBA and SBA were good markers for the hatching gland cells (HGCs) and mucous cells. Other lectins, such as PNA and UEA I, reacted only for a short time at some sites during the considered period of incubation. From 35 d until posthatching stages, a manifest strong reaction was noted both in the dorsal epithelium of branchial arches and the HGCs as shown by SBA reactivity. This may be significant with regard to the controversial origin of HGCs, which is thought to be endodermal.
本文报道了对孵化35至71天的褐鳟胚胎鳃区的组织化学研究。使用了一组6种不同的辣根过氧化物酶标记凝集素、PAS反应和阿尔辛蓝染色，来研究糖缀合物中碳水化合物残基沿咽和鳃上皮的分布。伴刀豆球蛋白A（Con A）和麦胚凝集素（WGA）在鳃区的每个部位都有反应，从而表明α-D-甘露糖和N-乙酰-D-葡糖胺普遍存在。WGA、大豆凝集素（DBA）和荆豆凝集素（SBA）是孵化腺细胞（HGCs）和黏液细胞的良好标记物。其他凝集素，如花生凝集素（PNA）和荆豆凝集素I（UEA I），在考虑的孵化期内仅在某些部位短时间反应。从35天到孵化后阶段，如SBA反应所示，在鳃弓的背侧上皮和HGCs中均观察到明显的强反应。这对于HGCs有争议的起源（被认为是内胚层起源）可能具有重要意义。
