Schick B, Habermann F, Sinowatz F
Lehrstuhl für Tieranatomie II, University of Munich, Munich, Germany.
Anat Histol Embryol. 2009 Apr;38(2):122-7. doi: 10.1111/j.1439-0264.2008.00908.x. Epub 2008 Nov 6.
A histochemical study using fluorescein isothiocyanate (FITC)-labelled lectins to identify glycoconjugates present in the efferent ductules and the three segments of the ductus epididymis (initial, middle and terminal segment) of dogs was carried out. The lectins used were: mannose-binding lectins (Con A, LCA and PSA), galactose-binding lectins (PNA, RCA), N-acetylgalactosamine-binding lectins (DBA, SBA, SJA and GSL I), N-acetylglucosamine-binding lectins (WGA and WGAs), fucose-binding lectins (UEA) and lectins which bind to complex carbohydrate configurations (PHA E and PHA L). The lectin-binding pattern in the canine epididymis presents similarities and differences to those observed in other mammalian species. The ductuli efferentes distinctly stained with most of the lectins used, whereas in the ductus epididymis a segment specific staining pattern was observed. Whereas principal cells of the ductus epididymis stained clearly with several FITC-labelled lectins (WGA, UEA and PHA-L), basal cells showed only a significant binding of Con A.
开展了一项组织化学研究，使用异硫氰酸荧光素（FITC）标记的凝集素来鉴定犬的输出小管和附睾管三个节段（起始段、中间段和终末段）中存在的糖缀合物。所用的凝集素有：甘露糖结合凝集素（伴刀豆球蛋白A、扁豆凝集素和豌豆凝集素）、半乳糖结合凝集素（花生凝集素、蓖麻凝集素）、N-乙酰半乳糖胺结合凝集素（大豆凝集素、荆豆凝集素、荆豆凝集素和麦芽凝集素I）、N-乙酰葡糖胺结合凝集素（小麦胚凝集素和小麦胚凝集素变体）、岩藻糖结合凝集素（荆豆凝集素）以及结合复杂碳水化合物构型的凝集素（PHA-E和PHA-L）。犬附睾中的凝集素结合模式与在其他哺乳动物物种中观察到的模式既有相似之处，也有不同之处。大多数所用凝集素可使输出小管明显染色，而在附睾管中观察到了节段特异性染色模式。附睾管的主细胞用几种FITC标记的凝集素（小麦胚凝集素、荆豆凝集素和PHA-L）染色清晰，而基底细胞仅显示出与伴刀豆球蛋白A有显著结合。
