Tran-Minh C
Ecole des Mines de Saint-Etienne, France.
J Mol Recognit. 1996 Sep-Dec;9(5-6):658-63. doi: 10.1002/(sici)1099-1352(199634/12)9:5/6<658::aid-jmr317>3.0.co;2-m.
This paper presents the construction of various biosensors using thin-film layers incorporated in flow injection devices, providing automated systems for biomedical analysis, process and environmental monitoring. A urease sensor has been developed in conjunction with a flow injection system for the automatic determination of urea. Use of the spraying immobilization technique gives rise to a response time of a few seconds, which allows sample throughputs up to 200 h-1. With a penicillin biosensor adapted in an appropriate cell detection, on-line measurements of penicillin V in the fermentation broth are achieved during the whole fermentation process; the results are compared with the HPLC method. Linearity, sensitivity and reproducibility of the biosensor are studied with regards to sample dilution in a stirred flow detection cell to provide optimal operating conditions. Measurements without any change in parameters are obtained during the whole fermentation process. Acetylcholinesterase sensors have been used in batch systems for the determination of pesticides, but they require large amounts of substrate. When those enzyme sensors are combined with flow injection systems, only small volumes (100 microliters) of substrate are injected into the carrier stream and an automated system can be obtained for continuous control of water quality.
本文介绍了利用集成在流动注射装置中的薄膜层构建各种生物传感器的方法，这些生物传感器为生物医学分析、过程和环境监测提供了自动化系统。已开发出一种与流动注射系统相结合的脲酶传感器，用于自动测定尿素。采用喷雾固定技术可产生几秒的响应时间，这使得样品通量高达200 h-1。通过在合适的细胞检测中采用青霉素生物传感器，可在整个发酵过程中对发酵液中的青霉素V进行在线测量；并将结果与高效液相色谱法进行比较。在搅拌流动检测池中，针对样品稀释情况研究了生物传感器的线性、灵敏度和重现性，以提供最佳操作条件。在整个发酵过程中，无需改变参数即可进行测量。乙酰胆碱酯酶传感器已用于批量系统中农药的测定，但它们需要大量底物。当这些酶传感器与流动注射系统结合时，只需将少量体积（100微升）的底物注入载流中，即可获得用于连续水质控制的自动化系统。
