Leskinen E, Pamilo P
Department of Genetics, Uppsala University, Sweden.
Hereditas. 1997;126(1):17-23. doi: 10.1111/j.1601-5223.1997.00017.x.
Ribosomal DNA (rDNA) region, including the 3' end of the 18S rRNA gene, the entire 5.8S rRNA gene, the 5' end of the 28S rRNA gene, and the internal transcribed spacers ITS 1 and ITS 2, of Enteromorpha green algae from the Baltic Sea, were sequenced. The evolution of the Enteromorpha sequences differed from those of other green algae in several important ways. The ITS regions were short and had a high nucleotide bias. The frequency of nucleotides G and C was up to 70% in the ITS sequences, whereas the frequencies were close to 50% in the 5.8S rDNA. Furthermore, the sequence divergence was much higher in ITS 1 than in ITS 2. Two haplotypes, differing only by two nucleotides, were detected in the E. intestinalis/compressa complex. The difference coincides with a morphological differentiation (branching of thalli) and may represent distinct gene pools.
对来自波罗的海的浒苔绿藻的核糖体DNA（rDNA）区域进行了测序，该区域包括18S rRNA基因的3'端、整个5.8S rRNA基因、28S rRNA基因的5'端以及内部转录间隔区ITS 1和ITS 2。浒苔序列的进化在几个重要方面与其他绿藻不同。ITS区域较短且核苷酸偏差较高。ITS序列中核苷酸G和C的频率高达70%，而在5.8S rDNA中该频率接近50%。此外，ITS 1中的序列分歧比ITS 2中的高得多。在肠浒苔/扁浒苔复合体中检测到两种单倍型，仅相差两个核苷酸。这种差异与形态分化（藻体分支）一致，可能代表不同的基因库。
